增加菌落计数的算法
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@ -9,6 +9,9 @@ using System.Linq;
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using System.Text;
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using System.Threading.Tasks;
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using System.Windows.Forms;
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using System.Threading;
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using System.Web.UI.WebControls;
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namespace PBAnaly.Module
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{
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@ -25,6 +28,12 @@ namespace PBAnaly.Module
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private Image<Rgb24> image_rgb_24 = null;
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private LanesImagePanel imagePanel = null;
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private BioanayImagePaletteForm imagePaletteForm = null;
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private PBBiologyVC.PBColonyVC pbvc = new PBBiologyVC.PBColonyVC();
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private Thread algThread;
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private bool isalgRun = false;
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private bool isUpdateAlg = false;
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#endregion
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public ColonyMannage(string _path, ReaLTaiizor.Controls.Panel _pl_right, Dictionary<string,ColonyMannage> colonyMannages)
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@ -43,11 +52,23 @@ namespace PBAnaly.Module
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return;
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}
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ImageAlg();
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RefreshImage();
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}
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#region 方法
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private void ImageAlg()
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{
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unsafe
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{
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fixed (byte* p = image_byte)
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{
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pbvc.run(p, 16, (ushort)image_L16.Width, (ushort)image_L16.Height, -1, -1);
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}
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}
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}
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private bool ReadTif()
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{
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// 读tif 或 tiff
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@ -1,53 +1,53 @@
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#include "pch.h"
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//#include "PBColonyVC.h"
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//#include <opencv2/opencv.hpp>
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//#include <PBColony.h>
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//
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//PBBiologyVC::PBColonyVC::PBColonyVC()
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//{
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// // pbcolony = new PBColony;
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//}
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//PBBiologyVC::PBColonyVC::~PBColonyVC()
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//{
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// //delete pbcolony;
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//}
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//
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//void PBBiologyVC::PBColonyVC::run(System::Byte* image, int bit, unsigned short width, unsigned short height,int lower=-1,int upper=-1)
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//{
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// //cv::Mat input_cn1;
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// //if (bit == 16)
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// //{
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// // input_cn1 = cv::Mat(height, width, CV_16UC1, image);
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// // cv::normalize(input_cn1, input_cn1, 0, 255, cv::NORM_MINMAX); // ¹éÒ»»¯µ½ 0-255
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// // input_cn1.convertTo(input_cn1, CV_8UC1); // ת»»Îª CV_8UC1
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// //}
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// //else if (bit == 8)
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// //{
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// // input_cn1 = cv::Mat(height, width, CV_8UC1, image);
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// //}
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// //else
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// //{
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// // return;
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// //}
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// //Mat input_cn3;
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// //Point2f center;
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// //float radius;
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// //int ret =pbcolony->colony_get_circle(input_cn1, center, radius);
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// //if (ret)
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// //{
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// // cv::Mat mask = pbcolony->generateMaskImage(input_cn1.cols, input_cn1.rows, center.x - radius, center.y - radius, 2 * radius, 2 * radius);
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// // int lower = -1;
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// // int upper = -1;
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// // Mat bin = pbcolony->get_lower_upper(input_cn1, mask, lower, upper);
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//
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// // ClassifyStandard class_stand;
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// // pbcolony->init_classify_standard(class_stand);
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// // vector<ColonyInfo> Cinfo = pbcolony->get_colony_info(input_cn1, bin, input_cn3, class_stand, pbcolony->image_inverted_flag);
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// // ColonyStatistic CStatistic = pbcolony->get_colony_statistics(Cinfo);
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//
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// // cv::imshow("a", input_cn3);
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// // cv::waitKey(0);
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//
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//
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// // }
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//}
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#include "PBColonyVC.h"
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#include <opencv2/opencv.hpp>
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#include <PBColony.h>
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void PBBiologyVC::PBColonyVC::run(System::Byte* image, int bit, unsigned short width, unsigned short height,int lower,int upper)
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{
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PBColony pbcolony;
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cv::Mat input_cn1;
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if (bit == 16)
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{
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input_cn1 = cv::Mat(height, width, CV_16UC1, image);
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cv::normalize(input_cn1, input_cn1, 0, 255, cv::NORM_MINMAX); // ¹éÒ»»¯µ½ 0-255
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input_cn1.convertTo(input_cn1, CV_8UC1); // ת»»Îª CV_8UC1
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}
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else if (bit == 8)
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{
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input_cn1 = cv::Mat(height, width, CV_8UC1, image);
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}
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else
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{
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return;
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}
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Mat input_cn3;
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Point2f center;
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float radius;
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int ret =pbcolony.colony_get_circle(input_cn1, center, radius);
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if (ret)
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{
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cv::Mat mask = pbcolony.generateMaskImage(input_cn1.cols, input_cn1.rows, center.x - radius, center.y - radius, 2 * radius, 2 * radius);
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int lower = -1;
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int upper = -1;
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Mat bin = pbcolony.get_lower_upper(input_cn1, mask, lower, upper);
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ClassifyStandard class_stand;
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pbcolony.init_classify_standard(class_stand);
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vector<ColonyInfo> Cinfo = pbcolony.get_colony_info(input_cn1, bin, input_cn3, class_stand, pbcolony.image_inverted_flag);
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ColonyStatistic CStatistic = pbcolony.get_colony_statistics(Cinfo);
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cv::imshow("a", input_cn3);
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cv::waitKey(0);
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}
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}
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PBBiologyVC::PBColonyVC::~PBColonyVC()
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{
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}
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@ -1,66 +1,77 @@
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//#pragma once
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//
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//using namespace System;
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//using namespace System::Collections::Generic;
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//using namespace System::Runtime::InteropServices;
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//
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//namespace PBBiologyVC
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//{
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// public enum dataClassVC
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// {
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// AREA = 0, //面积
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// PERIMETER, //周长
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// DIAMETER, //直径
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// IOD, //IOD
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// };
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// public ref struct ColonyInfoVC
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// {
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// int IDX; //菌落序号
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// int area; //菌落面积
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// int perimeter; //菌落周长
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// float diameter; //菌落直径
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// int IOD; //菌落IOD
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// int classify; //菌落分类
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// };
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// public ref struct ClassifyStandardVC
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// {
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// List<float>^ interval; //分类依据间隔标志数据
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// float maxd; //分类最大间隔标志
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// float mind; //分类最小间隔标志
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// int num; //分类间隔数
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// dataClassVC classes; //分类类别
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// };
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// public ref struct MinMaxInfoVC
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// {
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// float mind; //最小值
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// float minIDX; //最小值对应序号
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// float maxd; //最大值
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// float maxIDX; //最大值对应序号
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// float range; //范围
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// float mean; //均值
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// float sum; //和
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// int number; //数量
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// };
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//
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//
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// public ref struct ColonyStatisticVC
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// {
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// MinMaxInfoVC area; //面积统计结果
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// MinMaxInfoVC perimeter; //周长统计结果
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// MinMaxInfoVC diameter; //直径统计结果
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// MinMaxInfoVC IOD; //IOD统计结果
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// MinMaxInfoVC classify; //分类统计结果
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// };
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#pragma once
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using namespace System;
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using namespace System::Collections::Generic;
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using namespace System::Runtime::InteropServices;
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namespace PBBiologyVC{
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public ref struct ColonyInfoVC
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{
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public:
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int IDX; //菌落序号
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int area; //菌落面积
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int perimeter; //菌落周长
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float diameter; //菌落直径
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int IOD; //菌落IOD
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int classify; //菌落分类
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};
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public enum dataClassVC
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{
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AREA = 0, //面积
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PERIMETER, //周长
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DIAMETER, //直径
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IOD, //IOD
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};
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public ref struct ClassifyStandardVC
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{
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List<float>^ interval; //分类依据间隔标志数据
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float maxd; //分类最大间隔标志
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float mind; //分类最小间隔标志
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int num; //分类间隔数
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dataClassVC classes; //分类类别
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};
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public ref struct MinMaxInfoVC
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{
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float mind; //最小值
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float minIDX; //最小值对应序号
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float maxd; //最大值
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float maxIDX; //最大值对应序号
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float range; //范围
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float mean; //均值
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float sum; //和
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int number; //数量
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};
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public ref struct ColonyStatisticVC
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{
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MinMaxInfoVC area; //面积统计结果
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MinMaxInfoVC perimeter; //周长统计结果
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MinMaxInfoVC diameter; //直径统计结果
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MinMaxInfoVC IOD; //IOD统计结果
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MinMaxInfoVC classify; //分类统计结果
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};
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public ref class PBColonyVC
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{
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~PBColonyVC();
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public:
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void run(System::Byte* image, int bit, unsigned short width, unsigned short height, int lower, int upper);
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};
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//public ref class PBColonyVC
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//{
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// PBColonyVC();
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//
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// ~PBColonyVC();
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//
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// //PBColonyVC();
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// //~PBColonyVC();
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//public:
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// void run(System::Byte* image, int bit, unsigned short width, unsigned short height, int lower , int upper );
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//
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// //void run(System::Byte* image, int bit, unsigned short width, unsigned short height, int lower , int upper );
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//private:
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// //PBColony* pbcolony;
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//};
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//}
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}
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